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Cell Line Name：

Host Cell:

Ba/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

KIT K642E lies within the tyrosine kinase domain 1 (exon 13) of the Kit protein (PMID: 15990278). K642E results in constitutive phosphorylation of Kit and activation of downstream signaling (PMID: 15990278, PMID: 19802003), as well as transformation of cultured cells (PMID: 11073817).

1.Sanger of c-Kit K642E-D816A/BaF3

Figure 1. c-Kit_K642E-D816A/BaF3 K642E

Figure 2. c-Kit K642E-D816A/BaF3 D816A

2. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of BaF3 c-KIT K642E/D816A (C5).